Solid-phase refolding of poly-lysine tagged fusion protein of hEGF and angiogenin
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چکیده
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The homology of angiogenin and pancreatic RNase A provides a compelling reason to systematically compare the characteristics of the two proteins using the chemical modification approaches that proved essential to understanding the action of RNase. Reagents specific for histidine, lysine, and arginine markedly decrease the ribonucleolytic activity of angiogenin, much as has been observed for RNa...
متن کاملSynthesis and Phase Behavior of Poly(N-isopropylacrylamide)-b-Poly(L-Lysine Hydrochloride) and Poly(N-Isopropylacrylamide-co-Acrylamide)-b-Poly(L-Lysine Hydrochloride)
The synthesis of poly(N-isopropylacrylamide)-b-poly(L-lysine) and poly(N- isopropylacrylamide-co-acrylamide)-b-poly(L-lysine) copolymers was accomplished by combining atom transfer radical polymerization (ATRP) and ring opening polymerization (ROP). For this purpose, a di-functional initiator with protected amino group was successfully synthetized. The ATRP of N-isopropylacrylamide yielded narr...
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ژورنال
عنوان ژورنال: Biotechnology and Bioprocess Engineering
سال: 2002
ISSN: 1226-8372,1976-3816
DOI: 10.1007/bf02935871